Tea Triterpenoidal Saponins from the Roots of Camellia sinensis Have Inhibitory Effects against Alcohol Dehydrogenase

Abstract

Ten new polyhydroxyolean-12-ene pentacyclic triterpenoidal saponins, named rogchaponins 1–10, were isolated from the methanolic extract of the roots of Camellia sinensis by a series of chromatographic methods (silica gel flash column and C18 MPLC followed by C18 HPLC). Their structures were established by 1D and 2D-NMR techniques along with IR and HR-TOF-MS. Rogchaponins R4 (4) and R5 (5) showed inhibitory activities against yeast alcohol dehydrogenase (ADH) with IC₅₀ values of 16.1 ± 3.2 and 15.4 ± 3.3 µM, respectively. A 4-methylpyrazole positive control exhibited an IC₅₀ of 2750 ± 50 µM. However, the saponins showed no inhibitory activity against yeast aldehyde dehydrogenase (ALDH).

Key words

tea root - Camellia sinensis - Theaceae - triterpenoid saponin - rogchaponin - alcohol dehydrogenase

References

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